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KMID : 0380019940090050518
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Korean Journal of Biotechnology and Bioengineering
1994 Volume.9 No. 5 p.518 ~ p.523
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The Development of Serum Free Medium for the Economic Production of scu-PA from HEK Cells
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Abstract
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Serum free medium was developed to cultivate Human Embryonic Kidney cells with Cytodex III microcarriers. The cells normally attached and spreaded on the microcarriers in serum free medium, and grew well by bead to bead processes. 85% of attachment yield was obtained on Cytodex III in this medium, compared to about 93% in 1% serum containing medium. About 90% of the attached HEK cells spreaded after 6 hours of post-attachment periods on the surface of microcarrier. Maximum cell density and scu-PA concentration in a serum free medium were 9.1 x 10´ cells/ml and 1790 IU/ml, respectively, with fed-batch cultivation. Maximum cell density and scu-PA concentration in this medium with perfusion cultivation were 2.5X 106 cells/ml and 1820 IU/ml, respectively. The conversion of single chain urokinase type plasminogen activator(scu-PA) into two chain type plasminogen activator (tc-PA) was less than 5 % in a serum free medium compared to about 10% in 0.5% serum containing medium.
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KEYWORD
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